• Home
    • Testimonials
  • How it works
  • About
    • Technical documentation
  • Advantages
  • Contact
  • Press
  • Membership
  •   | FR |
LinkiLab LinkiLab
  • Home
    • Testimonials
  • How it works
  • About
    • Technical documentation
  • Advantages
  • Contact
  • Press
  • Membership
  •   | FR |

Technical Documentation

Chemical analysis: Size-exclusion chromatography

Principle : The size exclusion chromatography (sec), also known by gel permeation chromatography (gpc) , is a technique of liquid chromatography characterization that allows for the separation of biological macromolecules in solution according to their sizes (volume hydrodynamics) and their structures. it is based on the principle of sieving molecular.

Depending on their size, the molecules eluted may more or less penetrate into the pores of the stationary phase, a polymer gel which is filled in the columns. thus, the molecules the smaller ones are more retained than the larger, where a retention time longer for the first than for the second. each column has a domain of specific separation: the macromolecules of which the volume hydrodynamics is too important elected in the dead volume, while those that are too small elected with the eluent at the end of injection.

Interest/Objective:

It allows to obtain the different molar masses (mw , mn ,mp) and the index of polymolécularité (ip) of all types of macromolecules : proteins, natural polymers and synthetic polymers.

Ultraviolet-visible spectroscopy

Chemical analysis
7

Size-exclusion chromatography

Chemical analysis
4

Mass spectrometry

Chemical analysis
7

Gas chromatography

Chemical analysis
4

Liquid chromatography

Chemical analysis
6

Raman spectroscopy

Chemical analysis
7

X-ray fluorescence spectroscopy

Chemical analysis
7

Spectroscopy NMR

Chemical analysis
8
  • Chemical analysis: Size-exclusion chromatography
  • Available service providers at LinkiLab: +10
Connect to the online marketplace of research services
4
Share

Related Projects

Connectez-vous sur notre application et créez votre compte aujourd'hui. Allons-y !

  • LinkiLab
  • Lyon, France
  • +33 07 56 80 40 71
  • contact@linkilab.fr
  • https://www.linkilab.fr

• A propos
• Fiches techniques
• Conditions Générale d’Utilisation et de Vente

© 2023 LinkiLab | Mentions Légales | Legal

Prev Next